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石英とガラスのキュベットの比較: どちらを選ぶべきか?

The single most common キュベット question we answer every day. Here is the practical, no-fluff comparison — transmission range, price, durability and exactly when glass is (and is not) good enough.

1. The Short Answer

  • Measure below 320 nm (UV)? → You need 溶融石英. Glass blocks UV light.
  • Only visible 波長 (400–700 nm)? → Glass works, and costs a fraction of 石英.
  • Not sure? → The 波長範囲 of your methods decides. DNA/RNA at 260 nm, proteins at 280 nm and most pharmaceutical assays all sit below 320 nm — that is why most labs end up with 石英.
PropertyFused 石英 (JGS1/JGS2)Optical glass (K9 / borosilicate)
Transmission range190 – 2500 nm320 – 2500 nm
UV cut-off~170 nm (JGS1)~320 nm
Best forDNA/RNA, proteins, kinetics, HPLC, UV-VisVisible colorimetry, education
PriceHigherLow
Chemical resistanceExセルent (except HF/strong alkali)Good

2. Transmission Range: Why UV Decides Everything

Light absorption follows the 材質's transmission 窓. Fused 石英 (溶融シリカ) is transparent from ~190 nm in the deep UV through the visible to ~2500 nm in the near 赤外線. Borosilicate or optical glass (K9) cuts off sharply below ~320 nm — below that, the キュベット itself absorbs the light you are trying to measure, producing flat, meaningless readings.

Typical 波長 that force you into 石英:

  • 260 nm — DNA/RNA quantification (NanoDrop-style methods)
  • 280 nm — protein quantification
  • 230 nm — peptide bonds, many pharmaceutical methods
  • 200–220 nm — far-UV kinetics, nitrate/nitrite, many organic assays

If your lab only runs visible-range colorimetry (e.g. some water-quality tests, chlorine, copper), glass is a legitimate cost saver. Browse our glass キュベット for those workflows.

3. 材質 グレードs: It Is Not Just "石英 vs Glass"

石英 itself comes in グレードs, and knowing the difference avoids paying for performance you do not need — or missing performance you do:

グレードUV cut-offTypical use
JGS1 (far-UV グレード)~170 nmDNA/protein, anything below 220 nm — our 標準 for 10 mm セルs
JGS2 (UV グレード)~200 nmRoutine UV-Vis above 200 nm
JGS3 (IR グレード)Visible / NIRNear-IR, high-temperature work
K9 / borosilicate~320 nmVisible-range colorimetry only

For any biochemistry or pharmaceutical application, choose JGS1 far-UV グレード. The premium over JGS2 is small compared with the cost of one failed assay. All our 標準 10 mm 石英キュベット are JGS1 as 標準.

4. When Glass Is Perfectly Fine

Do not buy 石英 for the sake of it. ガラスキュベット are the right tool when:

  • All your 波長 are above 320 nm (visible colorimetry).
  • You teach or demonstrate — breakage is cheap to replace.
  • You need disposable-style 容積 workflows where cost dominates.
  • You are screening many samples and only need semi-quantitative results.

Our glass キュベット range covers 標準 10 mm セルs and specialty formats for visible-light work.

5. Price: The Real Cost Difference

A 標準 10 mm glass キュベット may cost a few dollars; an equivalent JGS1 石英 セル from a factory-direct supplier typically runs several times more, and ブランドed German equivalents cost substantially more again. However, the price gap has narrowed — factory-direct 石英 from manufacturers like Scuvette is often 30–70% below distributor pricing for the same JGS1 グレード.

Rule of thumb: if even one method in your lab needs UV, 標準ize on 石英. Glass セルs become a second set for visible-only work, not the default.

6. Durability, Cleaning and Chemical Resistance

  • Heat tolerance: 石英 withstands high temperatures (溶融シリカ up to ~1000°C continuous); glass softens far earlier. Relevant if セルs are autoclaved or used at elevated temperature.
  • Cleaning: both survive routine acid/base and solvent cleaning. Never use hydrofluoric acid on 石英 — it etches silica. Strong hot alkalis also attack 石英 over time.
  • Surface quality: 石英 is harder and less prone to fine scratches from routine wiping, which matters because scratched optical faces scatter light and raise baseline noise.

See our キュベットケアガイド for step-by-step cleaning and storage.

7. How to Tell Which キュベット You Already Have

Lab drawers are full of unlabelled セルs. Three quick checks:

  1. The UV test: measure empty-セル absorbance at 260 nm (air reference). 石英 reads near zero; glass shows a large, rising absorbance below 320 nm.
  2. The weight test: 石英 セルs feel noticeably heavier and denser than the same glass セル.
  3. The marking test: quality セルs are laser-marked (e.g. "Q" or "10 mm"); glass セルs are often unmarked or printed.

8. Decision Checklist

  1. Any measurement below 320 nm? → 石英 (JGS1 if below 220 nm).
  2. Visible colorimetry only, cost-critical? → Glass is acceptable.
  3. Autoclaving or high temperature? → 石英.
  4. Long-term reproducibility (matched pairs)? → 石英, matched to ±0.01 mm.
  5. Fluorescence? → 4-窓 石英 セル (glass autofluorescence ruins the reading). 蛍光キュベット →
  6. Uncertain about your instrument? → Check our complete 石英キュベット購入ガイド or send us your model.
Need a second opinion? Tell us your instrument model and measurement 波長 — our engineers will confirm which キュベット (石英 or glass, and which グレード) fits, usually within one working day. Contact Scuvette →

石英 vs ガラス キュベット — よくある質問

石英とガラスキュベットの主な違いは?

石英(JGS1溶融石英)は190–2500 nmを含む深紫外まで透過しますが、ガラスは約350 nm以下を遮断します。UVには石英を、可視光にはガラスをお使いください。UVカットオフの解説

いつ石英を使う必要がありますか?

約350 nm以下の測定(UV A280のタンパク質/DNA、多くの核酸・ペプチドアッセイ)には石英が必要です。タンパク質定量

ガラスは石英より安いですか?

はい — ガラスセルは安価で可視領域に適し、UVが関わる場合は石英が価値があります。ガラスキュベット

ガラスは近赤外に使えますか?

標準ガラスは近赤外まで透過しますが、石英より損失が大きくなります。石英は約2500 nmまで届きます。IR/近赤外キュベット

プラスチックキュベットは選択肢になりますか?

使い捨てPS/PMMAは可視カラリメトリに適しますが、UVや有機溶媒には不向きです。すべてのキュベット

石英はガラスより傷つきやすいですか?

溶融石英は化学的に耐久性があります。光学面を守るため、研磨剤を使わず優しく洗浄してください。洗浄プロトコル

同じサイズで互換性がありますか?

はい — 石英とガラスのセルは12.5 × 12.5 × 45 mmの形式を共有し、同じホルダーに収まります。寸法表